Thermotolerance of adenylylsulfate reductase fromThiobacillus denitrificans
نویسندگان
چکیده
منابع مشابه
The nitric oxide reductase of Paracoccus denitrificans.
The nitric oxide (NO) reductase activity of the cytoplasmic membrane of Paracoccus denitrificans can be solubilized in dodecyl maltoside with good retention of activity. The solubilized enzyme lacks NADH-dependent activity, but can be assayed with isoascorbate plus 2,3,5,6-tetramethylphenylene-1,4-diamine as electron donor and with horse heart cytochrome c as mediator. Reduction of NO was measu...
متن کاملGlutaredoxin function for the carboxyl-terminal domain of the plant-type 5'-adenylylsulfate reductase.
5'-Adenylylsulfate (APS) reductase (EC 1.8.99.-) catalyzes the reduction of activated sulfate to sulfite in plants. The evidence presented here shows that a domain of the enzyme is a glutathione (GSH)-dependent reductase that functions similarly to the redox cofactor glutaredoxin. The APR1 cDNA encoding APS reductase from Arabidopsis thaliana is able to complement the cysteine auxotrophy of an ...
متن کاملStructure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution.
The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5'-phosphosulfate, APS) reductase catalyzes reversibly the reduction of APS to sulfite and AMP. The structures of APS reductase from the hyperthermophilic Archaeoglobus fulgidus in the two-electron reduced state and with sulfite bound to FAD are reported at 1.6- and 2.5- resolution, respectively. The FAD-sulfite adduct was detected after so...
متن کاملIdentification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans.
EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split...
متن کاملIn Silico Characterization of Nitrate Reductase Involved in Gold Nanoparticles Synthesis in Pseudomonas Denitrificans
Various studies suggest the involvement of nitrate reductase in gold nanoparticle synthesis from different bacterial sources. The present study focuses on the in silico characterization of four subunits namely alpha, beta, gamma and delta of respiratory nitrate reductase enzyme using online tools and softwares in Pseudomonas denitrificans .CodonW and CAIcal server are used to perform codon usag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1989
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1989.tb03137.x